Photoaffinity labeling of the human red-blood-cell urea-transporter polypeptide components. Possible homology with the Kidd blood group antigen
نویسندگان
چکیده
منابع مشابه
At physiological expression levels the Kidd blood group/urea transporter protein is not a water channel.
The Kidd (JK) blood group locus encodes a urea transporter that is expressed on human red cells and on endothelial cells of the vasa recta in the kidney. Here, we report the identification in human erythroblasts of a novel cDNA, designated HUT11A, which encodes a protein identical to the previously reported erythroid HUT11 urea transporter, except for a Lys(44) --> Glu substitution and a Val-Gl...
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Urea transporter B (UT-B) is a passive membrane channel that facilitates highly efficient permeation of urea. In red blood cells (RBC), while the major function of UT-B is to transport urea, it is assumed that this protein is able to conduct water. Here, we have revisited this last issue by studying RBCs and ghosts from human variants with defects of aquaporin 1 (AQP1) or UT-B. We found that UT...
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The D antigen content of nucleated red cell precursors in human bone marrow was estimated using autoradiography and 125I-anti-D. D antigen first appeared in the pronormoblast, and the quantity of antigen progressively increased during red cell maturation. Maximal anti-D binding occurred on mature red blood cells. Pronormoblasts, basophilic normoblasts, polychromatophilic normoblasts, and orthoc...
متن کاملPolypeptide chains from human red blood cell membranes.
Human red blood cell membranes contain at least 17 polypeptides of differing molecular weight ranging from 27,000 to 220,000. Previous publications have reported higher values for the largest molecular weight species, but are in error owing to incorrect calibration of sodium dodecylsulfate-polyacrylamide gels with unreduced proteins. The two highest molecular weight species which constitute app...
متن کاملAn Overview on: The Red Blood Cell Histogram
The RBC histogram is an integral part of automated hematology analysis and is now routinely available on all automated cell counters. This histogram and other associated complete blood count (CBC) parameters have been found abnormal in various hematological conditions and may provide major clues in the diagnosis and management of significant red cell disorders. In addition, it is frequently use...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1993
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1993.tb18395.x